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NanoLogix Inc. Message Board

  • phloam131314 phloam131314 Jul 14, 2011 10:48 PM Flag

    Interview Transcript

    Here's a rough transcript of the interview at the following URL: http://www.pharmamanufacturing.com/multimedia/2011/019.html

    Paul: .... Pharmaceutical Manufacturing Magazine and talking today with Bret Barnhizer. Bret is the CEO for NanoLogix. Bret, thanks for being with us today.

    Bret: Oh, you're welcome Paul. I'm glad to be here.

    Paul: Bret, first of all tell us a little bit about your NanoLogix technology which is for rapid microbial detection, identification ... tell us about it, and how it might be applicable to the Pharmaceutical industry.

    Bret: Well, we have three types of technology that have to do with detection and identification for microbes. The original technology was what we called BioNanoChannel which has a small disc with two and a half million channels in it. And you would coat that disc for antibodies and then pass fluids through it to try to catch antibodies or antigens in whatever solution your were pouring through the disc. That's something we haven't spent a lot of time trying to develop further because we've got some, let's say finance..... some costs that are higher than a lot of industries want to bear. So with that in mind, we've moved on to ... well, we still keep that there for say, work with the EPA but we've moved on to BioNanoPore which is a thin permeable membrane sandwiched between two layers of agar in a petri plate, and then BioNanoFilter which is a bio-cellulose membrane that has, um ... it's utility is that it's an antibody coating and it reacts with whatever antigens are present in the samples that are put to it. Now, the BNP or BioNanoPore technology is a technology that's very good for detection of microbes ... say total-plate count .... which is essentially 55% of the petri plate use. But for identification ... for specific identification ... it doesn't lend itself to that unless the morphology of the microbe you detect is so unique that it's got a singular fingerprint ... say Anthrax, or something like that. The BNF does both detection, and then after a couple of buffering processes gives a spot-on antibody antigen reaction and identification. So that's gotten a lot of attention along with BNP.

    Paul: We talked a little bit about speed to results because these are, you know, rapid methods and what allows you to get those results quickly that would be obviously of interest to <inaudible> pharma QA/QC lab.

    Bret: Sure. Well, let's take the BNP first. The sandwich-membrane technology, that thin-permeable membrane ... what it's done ... it has ... or how it's configured ... it's got a thicker layer of nutrient agar underneath the membrane, and a thin film on top. And the sample's placed on that thin film of agar on top of the plate the same way any sample would be plated out on a petri plate, and the membrane provides a platform for transference of whatever's growing there as a micro-colony within say 3,4,5,6 hours. Transference is done after some empirically-determined time to a staining plate that comes as part of the kit. And if there are microbes growing on that membrane ... on that thin permeable membrane ... then after you transfer it to the staining agar, within 5 to 15 minutes the stain will wick up through the perms in the membrane and color the micro-colonies that are growing and so what's unique there is a tremendous time-savings to detection especially if someone's just interested in total plate count where the time is cut ... it could be up to 400% or more from traditional detection. Before this permeable membrane was developed and placed as a sandwiched membrane there was no way to view, no practical way to view micro-colonies and this gives a platform where we can do that and users can do that.

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    • continued:


      Paul: And what times are we talking about then and you're talking about the BNP technology which is good for detection. What are we talking about?

      Bret: Let's just say average six hours in most cases. Sometimes a little bit less. Sometimes a little bit more. It depends on what the standard growth rate would be for any type of bacteria. When Batelle had done some tests for Anthrax and Bubonic Plague, their Anthrax results with the BNP ... their standard results were normally 24 hours. With the BNP membrane they got results in 6 hours. Now with Bubonic Plague, or Yersinia Pestis the standard results are 48 hours ... and at that, with that 48 hour time we were able to cut the time to 24 hours, so we cut it in half ... which may not sound like much, but if one did have an outbreak of Bubonic Plague, then cutting the time in half can save ten-times as many people.

      Paul: Now you said that you had some interest from Pharmaceutical companies, from Proctor and Gamble Pharma, GSK, interest in the technology. What kind of things are they talking to you about?

      Bret: Yeah, what they've talked to us about is ... you know, we're not privy to all their detection processes for Q&A, or QA/QC, but they have said that any process that would bring in detection times for any bacteria in their manufacturing and lab processes would be a huge economic savings to them for anything under 24 hours, which with most of the bacteria they're dealing with, from what we've seen, we can detect those bacteria in much less than 24 hours.

      P: Can you talk a little bit about how you would differentiate yourselves from other technologies on the market.

      Bret: Oh, certainly. Well the big thing is ... well, let's compare us, for example, to PCR. Our technology only detects live cells. PCR detects DNA trace for any cells that are present ... whether they're live or dead. And, unless something's changed ... dead cells can't hurt you. Dead bacteria aren't a threat. And we've run into that quite a bit with some of the tests we've seen run with our technology. Because some of the confirmation of the presence of bacteria is done with PCR just to double-check ours and see how our times compare to those. And so that's the big thing ... is we detect viable bacteria.

      • 3 Replies to phloam131314
      • continued:



        Paul: What other developments do you have?

        Bret: Well the BNF ... there's a second type of technology that gives spot-on antibody and antigen reaction, and then with buffering it is only for live cells. And it can give results, from what we've seen, for the trails that are going on, or the trial that is going on at the University of Texas Health Science Center in Houston for Group-B Streptococcus in anywhere from ... well, they've seen results in as early as 2 hours. Dependable results in 6 hours .... where results are detection of the bacteria, specific identification of the bacteria, and determination of antibiotic sensitivity all within 6 hours. As opposed to 48 to 72 hours with the traditional methods that are dictated by the CDC. So we're very excited about that. Now, what we see for the future is researchers at one bio-defense company, and the aforementioned clinical trail that's going on for Group-B Strep in Houston, should have 3 to 5 papers published this year. And then we're also in a clinical trial for Group-B Strep in Italy with one of the ... probably the leading doctor for infectious diseases for OBGYN. And he believes that he'll be published this year also. So we should have multiple papers published in a number of journals. And I can't .... one's been accepted so far for publication in a very established US journal and I can't reveal that, right now, what the name is. But it should be out within a couple of months. So we see that, and then a lot of interest when we just had ... the NAVY's just submitted a request for an order for BNF kits, and that's a big deal to us, because little outfits like the NAVY don't need FDA approval for use ... just like the EPA ... we work with the EPA on developing a comprehensive water quality test kit for use in all 10 EPA regions, and once that developed they don't need any approval other than their own. So we're pretty excited about all that.

        Paul: Well we certainly hope that you continue to work with the drug industry as well, and we'll check back with you in six months or a year and see how things are going. Bret, thanks so much for talking with us today.

        Bret: You're welcome Paul.

      • <<Paul: Now you said that you had some interest from Pharmaceutical companies, from Proctor and Gamble Pharma, GSK, interest in the technology. What kind of things are they talking to you about?

        Bret: Yeah, what they've talked to us about is ... you know, we're not privy to all their detection processes for Q&A, or QA/QC, but they have said that any process that would bring in detection times for any bacteria in their manufacturing and lab processes would be a huge economic savings to them for anything under 24 hours, which with most of the bacteria they're dealing with, from what we've seen, we can detect those bacteria in much less than 24 hours.>>

        Did that tell us anything worth while ?

        And what is this

        <<but they have said that any process that would bring in detection times for any bacteria in their manufacturing and lab processes would be a huge economic savings to them for anything under 24 hours.....>>

        so what's holding them back from using our technology ? IF it's a specific validations then say once overcome that we would be a go with those companies. That would be the exciting quote in that part of the Transcript. They really need to be more informative, shareholders are waiting patiently and when they do talk it needs to be better than this.

      • <<Paul: Now you said that you had some interest from Pharmaceutical companies, from Proctor and Gamble Pharma, GSK, interest in the technology. What kind of things are they talking to you about?

        Bret: Yeah, what they've talked to us about is ... you know, we're not privy to all their detection processes for Q&A, or QA/QC, but they have said that any process that would bring in detection times for any bacteria in their manufacturing and lab processes would be a huge economic savings to them for anything under 24 hours, which with most of the bacteria they're dealing with, from what we've seen, we can detect those bacteria in much less than 24 hours.>>

        Since people can't get some positive going I'll help you guys out !
        HUGE ECONOMIC SAVING !!

    • Thanks Phloam.

    • powerstock and TF up at the same time....suspicious indeed. No, they are not the same person though seriously, i am just reaching at straws here. who cares any way.

      Coltatt. I hear you! I would be a sunshine pumper if the company had nothing! Bottom line is it does. Whether they can parlay that into multimillion sales year after year is the big question. TMC seems to like it. CDC was interested at a recent ASM. Hopefully TMC adopts tech and CDC concurs. Bottom line is that people can be stupid until it is too late to fully capitalize. Happens all the time and it really does not matter except to the ones that were smart enough to capitalize. The rest will live their mundane life as usual, it could have been, it should have been....but, but, but, whatever.... Yes, we were at higher levels, but we were also at lower levels. The key point is the company is progressing. Nobody can predict a stock price in the short run. We are still in the short run here for sure. History, company said this welches grape juice, company said that 500 kits to Africa, company said this, NATO, company said that 40 acres and a mule. Look past that. Good luck.

    • You have a great weekend also.

      Ironic, Fog Hat's Slow Ride just started playing on the Tube and it has been just that. But I still like the direction
      Eudius

    • if your bull had tits it would be a cow.

    • What BS was that Petro ?

      Do you believe there is a clinical trial from what is posted here in this thread ?

    • Bump..

 
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