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Sarepta Therapeutics, Inc. Message Board

  • mjruby20000 mjruby20000 Mar 21, 2006 7:34 PM Flag

    Morpholino Reduce Brain Tumor Invasion


    Morpholino Oligos reduce brain tumor invasion, and again show good pharmacokinetics:

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    • ""In summary, we developed a glioma-endothelial coculture model suitable for studying laminin-8 expression and its inhibition in vitro by antisense oligos. Morpholino proved to be efficient inhibitors of laminin-8 expression in cocultures. Antisense oligos to laminin-8 chains also significantly inhibited invasion of two different glioma cell lines in vitro. The results suggest that laminin-8 may play an important role in glioma invasion. Morpholino oligos may provide an efficient method to block laminin-8 expression for future therapeutic purposes.

    • I especially like this part from the link you posted (paragraph 6):

      "...The potential of antisense is widely recognized, but it remained largely unfulfilled since, until recently, the available oligos suffered from poor specificity, instability, and undesirable non-antisense effects [22, 23]. These problems have been largely solved by the new generation of antisense oligos that offer the promise of safe and effective therapeutics for various diseases including cancer [23, 24]. New-generation antisense oligos are being used in studies to find effective medications and treatments for many disorders, including viruses and cancers. By blocking a gene's effects in a laboratory setting, they enable researchers to study the gene, its control, and the interactions between gene products. Antisense technology is being refined not only for drug validation and diagnostic purposes but also for the development of future treatments for patients.

      The most promising types of oligos are Morpholino and peptide nucleic acid (PNA; they have nucleobases attached to a neutral "peptide-like" backbone) oligos [22, 24]. The new study used short strands of genetic code (Morpholino antisense oligonucleotides) to block the messenger RNA (mRNA) carrying the gene's "instructions." As a result, the gene's "protein product," laminin-8, was not produced and the invasiveness of glioma cells was significantly reduced. ..."

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