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Pacific Biosciences of California, Inc. Message Board

  • rekhaus rekhaus Jan 18, 2013 4:52 PM Flag

    Comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers

    Comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers

    Sentiment: Strong Buy

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    • I have been watching PacBio and I find more good news as I dig more...PacBio might put sanger based sequencing to rest and might become instrument of choice for complex, high GC genome. Also why generate lots of sequence on Illumina or IonTorrent when you can get near perfect genome with PacBio data.

      Sentiment: Strong Buy

    • Sequence generated by Ion Torrent, MiSeq and Pacific Biosciences technologies displays near perfect coverage behaviour on GC-rich, neutral and moderately AT-rich genomes, but a profound bias was observed upon sequencing the extremely AT-rich genome of Plasmodium falciparum on the PGM, resulting in no coverage for approximately 30% of the genome. We analysed the ability to call variants from each platform and found that we could call slightly more variants from Ion Torrent data compared to MiSeq data, but at the expense of a higher false positive rate. Variant calling from Pacific Biosciences data was possible but higher coverage depth was required. Context specific errors were observed in both PGM and MiSeq data, but not in that from the Pacific Biosciences platform.

 
PACB
4.96-0.15(-2.94%)Sep 23 4:00 PMEDT

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