very pleased to realze the great potential of pacbio in tissue HLA typing, where read length is critical
right now, the "golden standard" for it is still Sanger sequencing in hospitals. Roche is trying to push their 454 in this field, however, their reads length is just not long enough. the sequencing throughput requirement for HLA typing is actually not high. given the long read length, high accuracy and high speed of pacbio, I'm pretty sure it will become the new golden standard in this field in the near future. every hospital that has a transplant unit and all blood centers need to do HLA typing. the cost of buying and running a pacbio should not be a problem for them at all.