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Pacific Biosciences of California, Inc. Message Board

  • codyoot codyoot Sep 4, 2013 10:18 AM Flag

    New Data Release: Arabidopsis Assembly Offers Glimpse of De Novo SMRT Sequencing for Larger Genomes

    Advances in our chemistries, throughput, and read length are pushing the envelope in the way we tackle larger genomes. We recently sequenced the model organism Arabidopsis thaliana and produced a successful assembly solely using PacBio® data. The data set resulting from this sequencing effort and assembly using SMRT® Portal is now available via Devnet for anyone who wants to give it a test drive.

    A few stats on Arabidopsis and the assembly using PacBio sequence data:

    Genome size: 124.6 Mb
    GC content: 33.92%
    Raw data: 11 Gb
    Assembly coverage: 15.37x
    Polished Contigs: 540
    Max Contig Length: 12.98 Mb
    N50 Contig Length: 6.19 Mb
    Sum of Contig Lengths: 124.57 Mb

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