They started testing Dec. 3. I have not seen anything re who's instrument but I am betting CPHD.
Asphalt, so we are not all inundating Scott Clark with random messages, could you write and addendum to your request for information asking if they already had a Lite Cycler before they decided to test for MRSA?
I e-mailed Scott Clark again and asked him why they were using both machines. I also asked how long it took to get results from the Lite Cycler and if it took a while was it practicle to use for admission testing. We'll see if he responds.
Sounds great in theory but doesnt fly in the real world. In all admission scenarion with GE, lab people would be doing nothing all day but testing MRSA. With only 5% or so testing positive, it is also cost prohibitive. Better to batch with PCR instrument and then isolate the positives. GE is a niche product for patients who are already sick and need MRSA diagnosis fast. This is small market compared to screening.
So, back in 2004, the conversion rate from UK Pound to US$ was 1.85.
In 2004 the test cost $18.50. Maybe the price and time to result has come down over the years, I don't know, but for a three hour test, with suspected manual preparation, most likely batch testing, this seems inefficient.
Maybe they had an existing Light Cycler and decided to use it. Get familiar with GX and than convert in the future??????
The LightCycler Staphylococcus and MRSA Detection Kits for Methicillin Resistant
Staphylococcus Aureus (MRSA)
The LightCycler Staphylococcus and the LightCycler MRSA detection kits are 2 real-time polymerase chain reaction (PCR) assays for patients suspected of having a methicillinresistant
S. aureus (MRSA) infection or being a carrier of the organism. After sample
preparation, up to 3 hours are required on the LightCycler real-time PCR instrument.
Evidence in abstract form suggests that these kits can be used to identify MRSA-positive samples after an overnight broth step or direct from nasal swabs. Results using the LightCycler kits for mixed samples containing Staphylococcus aureus, coagulase-negative
Staphylococci (CoNS) and mecA will be equivocal and therefore further conventional testing will be required. Further data on analytical and clinical utility would be required before a full
assessment could be made.......
........Unit cost – Approximately £10 per reportable result. Many laboratories already have LightCycler instruments.
Using the Lite Cycler first and than confirming with GX seems cumbersome and time consuming.
My understanding of the benefit of PCR is to determine, in the shortest amount of time if the patient needs to have special isolation. So if this hospital tests first with Lite Cycler and confirms with GX, than this might take a very long time. Primarily because I assume the Lite Cycler is a batch maching.
If you have people coming into the hospital and want to know how to handle them so they don't have to sit in a waiting room or go away and than come back using two machines seems very inefficient.
I e-mailed Scott Clark yesterday and asked him who they were using for their MRSA screening. He answered me today with the following response:
"We use both Cepheid and Roche equipment. The sample is initially tested on the Roche Lightcycler for the presense of staph, and if positive the sample is run through the Cepheid GeneXpert."