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NanoLogix, Inc. Message Board

  • abig109 abig109 Jan 31, 2011 5:45 PM Flag

    PCR verses NNLX product

    Lately the PCR products keep poping up on this board and I just want to remind people of what NNLX has in its tool kit.

    <<<Detection of Live Escherichia coli Cells from Drinking Water

    [0052]A 100 ml sample of water was pressed through the device and SDU of the invention having a black non-fluorescent nitrocellulose filter. Two ml of freshly made 4-Methylumbelliferyl-Beta-Dglucuronide (0.1 mg/ml) fluorogenic substrate in distilled water was pressed through the SDU. All micro-channels were filled with the solution of fluorogenic substrate. The device was incubated for 20 minutes at 40 degrees C. After incubation, the device was untwisted and the micro-channel plate and filter were placed under a fluorescent microscope: 60.times.; .lamda. excitation: 340-380 nm; .lamda. fluorescence: 450 nm. Micro-channels containing live E. coli appeared as blue fluorescent dots. Micro-channels without cells or live cells of other species appeared as black dots. The same results performed by other rapid methods could only be reached after 6-8 hours.>>>>

    NNLX = 20 minutes
    PCR = 6-8 hours
    To get to the same exact results.

    If I was paying the bill I know which one I would pick!
    How's that for a K.I.S.S.


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