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Pacific Biosciences of California, Inc. Message Board

  • andyisss andyisss Jan 23, 2013 12:54 PM Flag

    A Closer Look at Accuracy in PacBio Sequencing

    By Jonas Korlach, Chief Scientific Officer

    Single Molecule, Real-Time (SMRT®) DNA sequencing achieves highly accurate sequencing results, exceeding 99.999% (Q50) accuracy, regardless of the DNA’s sequence context or GC content. This is possible because SMRT sequencing excels in all three categories that are relevant when considering accuracy in DNA sequencing:

    1. Consensus accuracy
    2. Sequence context bias
    3. Mappability of sequence reads

    Since there has been some confusion in the community about accuracy in SMRT sequencing, I would like to describe how our system performs and how such high accuracy is achieved. You can download the full perspective, complete with graphs and figures. What follows below is a summary of that document.

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