PacBio data produces much better results than only llumina!!!!!
PacBio data produces much better results than only llumina
We have published the first complete genome sequence of a Staphylococcus aureus strain assigned to clonal complex 12. The strain was isolated in a food poisoning outbreak due to contaminated potato salad in Switzerland in 2009, and it produces staphylococcal enterotoxin B.
The genome of Staphylococcus aureus KLT6 strain has been sequenced with illumina and PacBio technologies. The bacterial genome sequenced is a Staphylococcus aureus from a outbreak in Switzerland. The outbreak was traced back to a staphylococcal enterotoxin B (SEB)-producing S. aureus strain that was designated strain KLT6 and was assigned to CC12 and spa type t160.
The assembly and scaffolding has been done with Era7 Bioinformatics' AG7 approach and the annotation with the Era7 Bioinformatics' bacterial annotation system BG7. This article shows how adding a little of PacBio data to an illumina bacterial genome project much better results can be obtained. The results have been checked by means of optical mapping.
This work is a new success of Era7 Bioinformatics' methods for bacterial genomics projects (link). In addition, this work also constitutes a new successful step in our project NEXTMICRO, about NGS and outbreak management.