Rich Roberts discusses single-molecule sequencing technology with Biome reporter. part 2
Given that Pacific Biosciences’ SMRT sequencing has been subject to negative rumours, how did you come to realise that this technology is actually a valuable and accurate tool?
My original principle interest was in the methylation patterns as it seemed to offer the possibility of determining the recognition specificities of restriction modification systems in an extremely facile way. This turned out to be true and has yielded a plethora of new and interesting results. Along the way it became clear that this technology had much greater promise than the early scurrilous rumours suggested. A major reason that the community has not appreciated this is that very few of them have tried it. The original rumours put them off from buying the machines.