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Pacific Biosciences of California, Inc. Message Board

  • nimuma nimuma Sep 4, 2013 12:28 PM Flag

    This paper may also have done damage to PACB

    PacBio 16S reads are too error prone to be useful. The abstract alone sounds bad for PacBio sequencing.

    J Microbiol Methods. 2013 Aug 30
    Efficacy of a 3rd generation high-throughput sequencing platform for analyses of 16S rRNA genes from environmental samples.

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    • ha ha, nonsense paper, here is a comment from an expert:
      Lex Nederbragt ‏@lexnederbragt 2 Sep
      This 16S #PacBio paper needs to be redone with =2, =3, =4, =5, =6 ... passes of insert as CCS input, not =2

      • 1 Reply to codyoot
      • Lex Nederbragt answer to (J Microbiol Methods. 2013 Aug 30 paper) PacBio DevNet The SMRT developers' network. Open source software, data, documentation and tips for PacBio sequencing.
        News and Features
        Introducing the PacBio RS II, which still delivers the longest reads and 99.999% consensus accuracy, plus double the throughput of the original system.

        SMRT Analysis 2.0.0 is a pre-requisite for analyzing data from the PacBio RS II and SMRT Analysis 2.0.1 is a pre-requisite for analyzing data from the P4 enzyme. Please upgrade to take advantage of the following features:

        Microbial assembly using fully integrated HGAP in SMRT Portal
        Analyze bacterial methylomes with the P4/C2 polymerase/chemistry combination

        Streamlined reference sequence upload within SMRT Portal
        Modification identification of 6-methyladenosine (6-mA) and 4-methylcytosine (4mC)
        Watch the SMRT Analysis 2.0 webinar, P4 polymerase webinar, and HGAP genome assembly webinar (Best on Chrome) for an overview.

        Data Release Announcement:
        Dataset for Arabidopsis genome sequenced using only PacBio® sequence data and assembled with SMRT® Portal is available for download. For more information, please see the PacBio Blog entry.

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