ha ha, nonsense paper, here is a comment from an expert:
Lex Nederbragt @lexnederbragt 2 Sep
This 16S #PacBio paper needs to be redone with =2, =3, =4, =5, =6 ... passes of insert as CCS input, not =2
Lex Nederbragt answer to (J Microbiol Methods. 2013 Aug 30 paper) PacBio DevNet The SMRT developers' network. Open source software, data, documentation and tips for PacBio sequencing.
News and Features
Introducing the PacBio RS II, which still delivers the longest reads and 99.999% consensus accuracy, plus double the throughput of the original system.
SMRT Analysis 2.0.0 is a pre-requisite for analyzing data from the PacBio RS II and SMRT Analysis 2.0.1 is a pre-requisite for analyzing data from the P4 enzyme. Please upgrade to take advantage of the following features:
Microbial assembly using fully integrated HGAP in SMRT Portal
Analyze bacterial methylomes with the P4/C2 polymerase/chemistry combination
Streamlined reference sequence upload within SMRT Portal
Modification identification of 6-methyladenosine (6-mA) and 4-methylcytosine (4mC)
Watch the SMRT Analysis 2.0 webinar, P4 polymerase webinar, and HGAP genome assembly webinar (Best on Chrome) for an overview.
Data Release Announcement:
Dataset for Arabidopsis genome sequenced using only PacBio® sequence data and assembled with SMRT® Portal is available for download. For more information, please see the PacBio Blog entry.