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Pacific Biosciences of California, Inc. Message Board

  • nimuma nimuma Oct 23, 2013 10:42 AM Flag

    For $1000 deposit, nanopore welcoms users who need long read, simple worlflow, low cost and realtime analysis

    Their readlength is up to 100K, and accuracy 96% (from their early claims).
    Bye bye pacbio, Ch 7 is coming. Just like old pal helicos.

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    • If their platform is that good, why Nanopore never released and data. This device might be good for sensing experiments (RNA etc), not for sequencing. We will wait and see...

    • Alotroladocr, I assume you nothing about sequencing. You don't even know the difference between throughput and readlength. By your definition of readlength, IlMN's HiSeq will have readlength of 600,000,000,000.
      I suggest you sell your house and take all your savings to buy PACB. But don't mislead others here by your erronous and ignorant post.

    • If you're going to bash a stock, at least make an attempt to perhaps sound like you know what you're talking about.
      Here is a Wikipedia definition:
      A kilobase (kb) is a unit of measurement in molecular biology equal to 1000 base pairs of DNA or RNA.[4]...

      Here is a paragraph from your Oxford Nanopore PR:
      The company did not say what the expected performance of the MinIon sequencer will be, but in 2012, when it first revealed the MinIon and GridIon platforms, it said that both systems are expected to deliver read lengths of up to 100 kilobases and raw read error rates of about 1 percent at launch. According to In Sequence, the firm said then that the disposable MinIon would have about 500 nanopores and generate a couple of hundred megabases per hour, with run times up to six hours.

      PACBIO's sequencer read is up 10,000 bases which sums up to 10,000,000 kilobases. Your Oxford Nanopore device is a lego block compares to PACBIO's system.

      NOW GO SLEEP. YOU'RE TIRED!!!!!!!!!!!!!!!


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