Prana BIotechnology is reporting results of thei lead drug PBT2 in March. This may be one of the biggest biotech opportunities to dat if results are posititive.
PLoS One. 2014 Feb 28;9(2):e90070. doi: 10.1371/journal.pone.0090070. eCollection 2014.
Neuroprotective Copper Bis(thiosemicarbazonato) Complexes Promote Neurite Elongation.
Bica L1, Liddell JR1, Donnelly PS2, Duncan C1, Caragounis A1, Volitakis I3, Paterson BM2, Cappai R4, Grubman A1, Camakaris J5, Crouch PJ6, White AR6.
1Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
2Bio21 Molecular Science and Biotechnology Institute, Parkville, Victoria, Australia ; School of Chemistry, The University of Melbourne, Melbourne, Victoria, Australia.
3Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia.
4Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia ; Bio21 Molecular Science and Biotechnology Institute, Parkville, Victoria, Australia.
5Department of Genetics, The University of Melbourne, Melbourne, Victoria, Australia.
6Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia ; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia.
Abnormal biometal homeostasis is a central feature of many neurodegenerative disorders including Alzheimer's disease (AD), Parkinson's disease (PD), and motor neuron disease. Recent studies have shown that metal complexing compounds behaving as ionophores such as clioquinol and PBT2 have robust therapeutic activity in animal models of neurodegenerative disease; however, the mechanism of neuroprotective action remains unclear. These neuroprotective or neurogenerative processes may be related to the delivery or redistribution of biometals, such as copper and zinc, by metal ionophores. To investigate this further, we examined the effect of the bis(thiosemicarbazonato)-copper complex, Cu(II)(gtsm) on neuritogenesis and neurite elongation (neurogenerative outcomes) in PC12 neuronal-related cultures. We found that Cu(II)(gtsm) induced robust neurite elongation in PC12 cells when delivered at concentrations of 25 or 50 nM overnight. Analogous effects were observed with an alternative copper bis(thiosemicarbazonato) complex, Cu(II)(atsm), but at a higher concentration. Induction of neurite elongation by Cu(II)(gtsm) was restricted to neurites within the length range of 75-99 µm with a 2.3-fold increase in numbers of neurites in this length range with 50 nM Cu(II)(gtsm) treatment. The mechanism of neurogenerative action was investigated and revealed that Cu(II)(gtsm) inhibited cellular phosphatase activity. Treatment of cultures with 5 nM FK506 (calcineurin phosphatase inhibitor) resulted in analogous elongation of neurites compared to 50 nM Cu(II)(gtsm), suggesting a potential link between Cu(II)(gtsm)-mediated phosphatase inhibition and neurogenerative outcomes.
Cools , The great science behind PBT2 is what will help people affected by this horrible disease in my opinion. pran = life force. Respect.
Prana has performed PIB/PET scans on patients in the IMAGINE trial. I am excited if that will show the clearance of Amyloid from the brains of this patients. Lipitor for the brain he said!
Thanks Pivalde, and the evidence from sources other than Prana supporting their Metals Theory keeps mounting,
Prana Biotechnology has now arrived and the Metals Theory is moving front and center. Very Exciting times to be and investor in this special biotech. Onwards and upwards to trial results.
Metallothioneins and the Central Nervous System: From a Deregulation in Neurodegenerative Diseases to the Development of New Therapeutic Approaches.
Bolognin S1, Cozzi B2, Zambenedetti P3, Zatta P4.
1Deparment of Neurological, Neuropsychological, Morphological and Motor Sciences, Section of Physiology, University of Verona, Verona, Italy.
2Department of Comparative Biomedicine and Food Science, University of Padova, Italy.
3Phatology Division, General Hospital, Dolo-Venezia, Italy.
4CNR-Institute for Biomedical Technologies, "Metalloproteins" Unit of Padova, University of Padova, Department of Biology, Padova, Italy.
Metallothioneins (MT) are a family of proteins actively involved in metal detoxification and storage as well as in prevention of free-radical damage. Changes in the levels of MT have been described in a number of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, prion protein disease, Binswanger type of subcortical vascular dementia, and amyotrophic lateral sclerosis. This suggests that MT functions might be more complex and vast than what was initially thought. In this review, we summarize the current knowledge on the potential involvement of MT in the mentioned neurodegenerative diseases while also discussing the emerging evidence proposing MT modulation as a feasible therapeutic approach. Enhancing repair mechanisms after neurological damage and/or protection against oxidative stress through a proper modulation of this family of protein might indeed represent an important avenue to cope neurodegeneration.
Brain development, metal ions, metallothioneins, neurodegenerative diseases
Pete, The truth is what Prana is about to announce to the world will blow your socks off in my opinion. I would urge you to Join us now in profiting on what will be a beautiful gift to mankind.
, I invested in Prana based on what in my opinion is their great science . The metals theory has received support from independent papers from such institutes as the Susan Lindquist Lab, Max Planck and many more. This along with Prana's own papers and previous trials is why people are investing in this exciting biotech.