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Alimera Sciences, Inc. (ALIM) Message Board

maryland944 1 post  |  Last Activity: May 6, 2014 2:40 PM Member since: May 6, 2014
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  • Purpose:
    The purpose of this work was to exploit the tunable
    properties of BioSilicon (a form of oxidized porous silicon) as a
    substrate to provide long term release Bevacizumab (Avastin).
    BioSilicon can be manufactured with varying pore size and high
    surface area. Proteins including antibodies, adsorb by a combination
    of electrostatic, Van der Waals, and hydrophobic forces, while
    changes in surface hydration, entropy, and protein structure (shape
    and size) are also important. Strong electrostatic forces on the surface
    not only enable the antibody to adsorb but also subsequently desorb
    from the surface. In this work we evaluated the effect of pore size on
    Avastin release over a period of three weeks

    Methods:
    Oxidised porous silicon (OPS) was prepared with pore
    sizes ranging from of 10 to 100nm (the hydrodynamic radii of
    Avastin monomer estimated to be ~ 14.52nm). 5mg of OPS was
    added to 25μL of a 25mg/mL Avastin stock solution (prepared from
    the commercial formulation) and allowed to equilibrate overnight
    at room temperature (22.5οC). 1975μL of Phosphate buffer saline
    (PBS: pH7.4; 37οC) was added to the equilibrated OPS particles.
    The suspension was then hand mixed for 10 seconds and centrifuged.
    1mL of PBS was taken to calculate post loading concentration and
    1mL of PBS was freshly added to the dissolution pot. Sampling
    was performed every 24hrs for 20 days. Samples were analysed by
    Size-Exclusion Chromatography coupled with UV and fluorescence
    detectors.

    Results:
    Maximal adsorption was obtained with OPS of average pore
    size 25 to 30nm which provided approximately 12% (w/w) loading,
    larger pores gave slightly less loading (10% at 50nm and 8% at
    100nm) due to lower surface area. Material with a pore size of 10nm.

    Sentiment: Hold

ALIM
5.76-0.11(-1.87%)Jul 24 4:00 PMEDT

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