Mark, I have never seen so many bashers here!!! Something big is on the horizon. This is worse than the run up from 0.75 to 3.00. I am going to be moving lots more money here in the near future because this is ridiculous... Did you see the Pompano's surfer club? Wow. He's trying to make waves somewhere since he isn't getting any at the beach...
I've said it a few days ago. They have at least 10 studies PUBLISHED that there is no PR for (yet). They are saving it for a big reason. I think they have waited for the stock to consolidate to push it through the next level.
I know I am not supposed to respond to bashers, but this is more about this bashers name... Really, pompanobeach surfers society? There are no waves in pompano beach. Do you sit around waiting for a wave to hit for a few days? It looks like your choice of investments is in line with your choice of place to live for surfing... Sitting around to try make money shorting INO. Dum du dum dum....DUMMMBBB.
It did it again. Way to go with the censorship.
My point is DNA vaccine + electroporation + IL-33 is much better than DNA vaccine with electroporation which is much much much much much much better than DNA vaccine alone (no IL-33 and no electroporation.
The real advance of INO when it comes to DNA vaccines is the electroporation and the Syncon, where they can actually tell the proteins encoded by the vaccine where to go. So for the HPV vaccine, I am sure they want the HPV proteins to build up as much as they can inside the cell before the cell dies. Whereas they want the cell to release the fully formed IL-33 so it works as an "alarmin" to attract immune cells there. So my stance is, yes IL-33 will definitely boost the immune response to the DNA vaccine. Could have other cytokines been just as good or better? Yes. Could have other cytokines been worse? Yes. But IL-33 would definitely get the job done if DNA vaccine by itself does not (which I don't believe will happen.
Their approach is still the reason this is my second largest investment. But having that last sentence of my original post that was originally cut off would have put my first post in perspective.
I believe any adjuvant would help. DNA vaccination itself is its own adjuvant. The DNA plasmid itself uses host machinery to replicate... Just like what a virus does. The host cell then recognizes the plasmid DNA, just like it would recognize a DNA virus (herpes, Hep B, chicken pox, small pox are all DNA viruses). The most important immune factor to fight against viruses is type I interferon (interferon alpha and beta), and DNA vaccination is quite efficient at activating this component of immunity. The problem is before electroporation, DNA vaccination couldn't find its way into the cells. With electroporation it can get into cells and do what its supposed to.
Now what does IL-33 do? It was originally found to be important in allergic inflammation (eczema, asthma, and hay fever). However, it also is released as cells are dying and acts as an "alarmin," to signal innate immune cells to come to the site of injury/infection/etc. A more recent paper in the journal Science showed IL-33 can also participate in antiviral immunity. I'm not quite sold on that. Anyhow, despite my opinion that IL-33 is not the best antiviral adjuvant, it is still better than no IL-33.
So DNA vaccine alone
My thoughts and prayers go out to you and your family. That is the most heartwrenching piece of news I can think of, and I'm sorry for your loss.
Rante... Thanks for all your DD.
If I can ask you for IGXT. Still more upside possible or is the run up done? FDA decision in very early February. Thoughts?
The reason I didn't create a new post/thread with this PR about IL-33 is because I wanted everybody to see the date.
This new IL-33 PR has been known for quite some time and is old news to the scientific community. See my posts below for my analysis in response to martha.varias post, and you will have your answer.
I think Dr. Kim is ready for the next leg up in increasing shareholder value. I think there are at least 10 PRs that can be released at any time, since the last major round of PRs.
Again, none of it is new. It has been in INOs back pocket. They wisened up from the last time and waited to consolidate completely rather than get a large pump and dump effect.
If anybody wants to copy my posts from below and paste it in a new thread, you are welcome to.
Hmmm... I guess all the shorts are gonna cover tomorrow? They were really hoping they could scare people. Looks like shorts couldn't blitzkrieg the Germans...
Was there any news on the partnership with Roche (did they scale up the partnership), or is this the same partnership from 2012? Thanks in advance.
His answer was actually mostly correct. Cut Modajon some slack. He's obviously not an immunologist, but he obviously did some DD to educate himself on one of his investments. Not a bizarre mechanism. TAP1 and TAP2 and invariant chain are the proteins that use the "active" mechanism to "load" antigen onto MHCI molecules. The peptide of interest replaces invariant chain, then the completed MHC molecule can be presented with the peptide on the surface of the DC. CD8 T cells use their T cell receptor to bind to the MHCI molecule.
And it does "go on all the time." Do you think you have 10s of millions of cells replicating on a daily basis and none of them get mutated? You think the replication efficiency of cells is 100%? I wouldn't say that a cell turns into cancer every day, but I'd say everybody gets mutated cells and your immune system recognizes it before it becomes a cancer. Heck the sun mutates cells when you go outside.
Good job Modajon. Very good layman description until the last paragraph. DCs don't identify mutant proteins, they are the ones presenting the mutant proteins on their surface to T cells. Initially this happens in lymph nodes after the DCs gobble up dead cells/proteins/parts in tissue and migrate back to the node. That's where they meet the T cells that are ready to fight something they don't like.
Now to answer your question: The key is central tolerance and peripheral tolerance. T cells developing in the thymus when you are a fetus up until you are a few months old are presented all of the antigens or protein components that your body makes by the thymic epithelial cells. Any T cell that binds too strongly to a presented antigen gets deleted- central tolerance. Now some proteins escape, and that is a mechanism you can possibly get autoimmune disease. Cancer proteins, since they are mutated were never seen by the T cells you have in your body, hence, these cancer proteins are "foreign" and can be attacked by your T cells, whereas your normal proteins from normal cells where Direct is injected (although picked up and processed the same way) will never activate your T cells, because the ones that could react to the normal proteins have been deleted a long time ago.
I will spare the peripheral tolerance lecture for another time as it is not pertinent to the original question on the topic. I am not invested in this company. As an immunologist, I find all immunotherapy companies interesting, and I am hopeful that immunotherapies will work sooner rather than later, for the sake of patients. Now quit soft bashing redhotserver.
I still follow him. You should too. The reason being, the stocks he bashes are GREAT STOCKS. You know after his idiot short followers do their thing, you can invest with no worries and gain 20-30% without worries. After he bashed INSM, I invested there. It went from 11 to now almost $22. Can't beat that strategy. Look at CYTR, he bashed when it hit 7. Down to 5. Now back up to 7. He bashed it again. But guess what? No effect. Because people realize its a great stock, with great data.
Good luck to all. But I think that may be an excellent investing strategy. I am just waiting for EXEL to drop like a rock when it fails whatever its doing. This is because it is a stock that AF is promoting.