On days like that was when a company should keep a PR in their arsenal. Volume was 30 million with ~15 million short. If they had the smallest PR of good news, all of those 15 million shorts and the 38% shorts from the week before that were concertedly keeping the stock down the whole week prior, would have been in for the world's biggest short squeeze. Over the past 10 days, over 500 million shares have exchanged hands. The demand is there.
If you or anyone have a (legitimate) scientific question. send me an email. Just say "INO question" in the subject line or something like that. I don't come here too often anymore. This stock is gonna do what its gonna do (go up), and I don't need to wade through 1000s of negative bull crud posts to see that.
Now cancer: In the original data it was found that hTERT EXPRESSION can be detected in up to 85%-90% of cancers. Mutations in hTERT are not found in 85-90% of human cancers. So cancers, through various mechanisms, can reactivate hTERT, just like B and T cells.
Now I cannot tell you the percentage of the 85-90% of cancers that have increased hTERT expression due to mutation of the hTERT gene. That has never been investigated. However, the locations of the mutations in hTERT have been identified, and most of them are in the same area of the hTERT gene. From a review by Joshua D. Podlevsky in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis in 2011 “When mapped onto the amino acid sequence, these TERT mutations are located almost exclusively within the conserved functional domains, with the majority concentrated within RT motifs.” The reason why I note this, is because the Inovio hTERT vaccine covers two mutations in the hTERT gene. From Inovio’s website “Inovio has developed a highly optimized synthetic hTERT DNA vaccine with two mutations designed to target multiple cancers expressing the antigen hTERT, including non-small cell lung carcinoma, breast cancer, melanoma, and prostate cancer.” It is important to note this because if you are targeting the endogenous, normal protein, you may kill normal stem cell populations such as hematopoietic stem cells resulting in aplastic anemia. That would be no bueno. INO is smart, and they will be able to kill a large percentage of cancers that have mutations in the hTERT gene without affecting normal cells that depend on hTERT to keep us alive. Other companies are using the endogenous hTERT as a dendritic cell vaccine. Good luck with that. I don’t want to be one of the patients in that trial, even if I have cancer. I would take Inovio’s vaccine over that any day of the week. And they can combine this vaccine with specific tumor antigen vaccines (prostate, cervical, melanoma) as well.
So there are mutations in several of the telomerase genes that occur in humans. These include dyskeratosis congenital (mutation in dyskerin or TERC, two regulatory components of the functional complex), and these patients develop cancer at an early age (oral cancer, myelodysplastic syndrome, and leukemia) but die of lethal aplastic anemia when their bone marrow fails. Also aplastic anemia, is another disease that is thought to be caused by inactivation/mutation of telomerase (remember above where I said hematopoietic stem cells have activated TERT expression).
OK, so this is a bit of a touchy subject, and one where the company isn't 100% accurate on. This is not any fault of their own, but its more a misquote of the scientific literature that has been repeated (and changed along the way) so many times that it became a new truth… Sort of like when we played telephone when we were kids. Background: Human telomerase reverse transcriptase (hTERT) is an enzyme that adds telomeres, or short repeats of DNA to the end of chromosomes after a cell divides (through a process that I have not studies, so I don’t know the exact mechanism). This is done because every time a cell divides or replicates, it has to make a copy of its DNA for the next cell. Each time it does that, a little portion of the extra telomere end DNA gets cut out. If telomeres get too short, cells die, even when cells are dividing. This occurs because DNA repair mechanisms get activated, but the repair mechanisms aren’t able to fix the DNA (you can’t fix not having enough DNA), the DNA present isn’t enough to make a full genome, so as a mechanism to protect the integrity of its genome (and prevent cancer from developing) these cells die by programmed cell death (or apoptosis). This gene is activated when we are embryos, but shut down soon after, and then we have our telomeres for life in all somatic cells. This is a mechanism of cellular senescence and one reason some people believe we age and die. Gonad telomerase is supposed to be active throughout life, and dedicated stem and progenitor cells (such as in the intestine and hematopoietic stem cells) do express telomerase as well. T cells and B cells (which replicate very quickly after spotting an infection) also can reactivate telomerase when needed.
I set my ask price too low today for my attempt to get my last 40,000. I couldn't raise it because I was at work.
I just got paid so I am transferring more money into my account. Hopefully it gets uploaded into my account. I am going to try to get another 80k shares. We'll see how it goes.
Yeah. My cost average was 1.48. So now I have doubled up again (When it hit 3 the first time, I originally doubled up, but it took a while to work back to this level again... Way longer than I thought. Like I said many times, INO is gonna pay for my kids college education (and possibly more if it goes parabalic with any luck). My shares are locked in. Looking for a pullback to add more in the future.
Been a while since I posted. Just wanted to say congrats. I'm now back up to doubling up. This time next year, what do you guys think? Five or ten bagger? How many more preclinical trials will be PR'ed after Phase II trials show incredible results. They are still doing the studies. They are still publishing them. They just aren't PR'ing them since they realized its falling on wall street's deaf ears.
Happy Holidays to all. We got a late Christmas present from Dr. Kim. Better late than never though!!! Thanks Dr. Kim
The patent already existed from Dr. Urano. All they did was acquire the license to the patent from them. Probably didn't cost them anything, and won't cost them anything until they decide to do the studies to test out MANF as a diagnostic. But Dr. Urano won't make any money off of that either. Dr. Urano will probably get a licensing fee if the test goes to market, and a percentage off of each test sold. So at this point its a win win for everyone. But if I was management, I wouldn't pursue it until they are making money with LymPro, and hopefully not until after MANF undergoes trials as a therapeutic for RP, or Wolfram's, or stroke, or Alzheimer's, or cardiac preservation after myocardial infarction, or prevention of type I diabetes, or .... well you get my drift...
Risk, Not Zoombboom, but I wrote these comments in another thread, so since you are asking I put my 2 cents here as well. I am not bashing AMBS, I am not bashing MANF (MANF is the reason why I am so bullish on this stock), I was just saying, at this point, I do not want them to pursue MANF as a diagnostic. They already have LymPro and NeuroPro. I want them to use MANF to treat some diseases... That's where the real power of this company is. You know how I like to keep it real.
Happy holidays to you and yours as well!!! Doing well, had some good R&R during Christmas, and ready to grind again. How bout yourself? Are you still in CPRX and INO as well?
I am not bashing AMBS, I am not bashing MANF (MANF is the reason why I am so bullish on this stock), I was just saying, at this point, I do not want them to pursue MANF as a diagnostic. They already have LymPro and NeuroPro. I want them to use MANF to treat some diseases... That's where the real power of this company is.
Thanks, zoombboom. I actually did see that PR. I understand that in the PR it says that they received the license to use MANF as a diagnostic and therapeutic in Wolfram's, type I, and I believe type II diabetes (if I am not mistaken). It would be a tough sell to make it into a clinical lab test for a specific disorder, and I can give my reasons why:
-It is a pleiotropic protein present in the ER of many cells.The problem is, any condition of cellular stress such as ischemia, hypoxia, tissue or cellular injury, starvation, certain medications, cancer etc, can likely lead to MANF release by cells, as an adaptive host response to the stress. Heck possibly even an intense workout may increase MANF levels, who knows...
-There are many stressors out there, and cells will induce MANF to protect themselves. It won't be specific for diabetes per se, so I don't see it achieving the specificity needed to being a diagnostic test.
-With Wolfram's, it is inherited from your parents, so if it runs in the family, and you get diabetes, you pretty much know you have the disease, so a diagnostic test is a moot point. However, if it can predict disease progression or severity of the disease, it can definitely be used in that regard.
-With Type II diabetes, patients in that age group will likely have other comorbidities raising MANF levels nonspecifically.
-The only place where there is possibility as a diagnostic is in children, where you are suspecting type I diabetes. An otherwise healthy child should not have elevated MANF levels, whereas a child with type I diabetes with subclinical attack on the beta cells in the pancreas may have increased levels. Like I already outlined above, the only other place would be to monitor disease progression (which 1: would be used multiple times to make sure levels aren't going higher, and 2: May actually be expanded as a non-specific marker of tissue injury, etc, such as C reactive protein is a non-specific marker of inflammation used daily) .
and another red day, would have sufficed. Another red day in a row falls under the jurisdiction of the Department of Redundancy Department.
Barons should we take our chart "reading" advice from someone whose grammar indicates he can't read at a middle school level? He almost scared me into immediately selling immediately.
I had a similar post this am. They were only able to take it down 10-11 cents with the bear raid. I thought with the bear raid they may be able to get it down to 7, lowest 6.80, but it held at 7. The bear raid may continue tomorrow, but come the new year, this is gonna take off.
I've been laying low (been crushed at work). Bashers just make me too mad, so I mostly just search for people's comments that I enjoy reading (the banter between Risk and Watson/Coldcoffee, etc. I went in big on ARIA, added more here, and INO, of course. Those are my big 3 for 2014. CYTR and NVIV are now on the watch list as well with small feelers out. Happy Holidays to you and yours