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Hutchinson Technology Inc. Message Board

this_isnt_my_real_alias 36 posts  |  Last Activity: 20 hours ago Member since: Dec 28, 2008
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  • this_isnt_my_real_alias this_isnt_my_real_alias 20 hours ago Flag

    Rest of Article:

    indicating that they have k-mers in common; close matches are overlapped to assemble the whole genome.

    According to the authors, this process is so fast that an E. coli genome can be assembled de novo using MHAP in essentially the same amount of time it would take to create a reference-guided assembly of the same genome from short Illumina reads. This would put de novo assembly within reach for small labs that previously only had access to the computing resources for reference-guided assembly. MHAP sees even greater gains in efficiency with larger, eukaryotic genomes.

    The authors used MHAP for assembly of a human genome, plus genomes of the bacterium E. coli, the yeast S. cerevisiae, the fruit fly D. melanogaster, and the plant A. thaliana. All these assemblies will be made freely available in GenBank. While none of the assemblies has yet been thoroughly vetted, the authors suggest that their assemblies may have closed gaps in the D. melanogaster, A. thaliana and even human reference genomes, thanks to their ability to use long-read sequencers. The most significant benefit of MHAP, however, is the time and cost savings to genomics labs. Both the D. melanogaster and A. thaliana genomes were assembled on a desktop computer in a matter of days; compared to an assembly of the fruit fly genome using the previous alignment tool BLASR, MHAP

  • By Bio-IT World Staff

    August 15, 2014 | This Thursday, a team of bioinformaticians from the National Biodefense Analysis and Countermeasures Center, the University of Maryland College Park, and sequencing company Pacific Biosciences posted information on their tool MHAP to the life sciences preprint server bioRxiv. MHAP, or MinHash Alignment Process, is a dramatically faster method for ordering DNA fragments sequenced on long-read technologies like the PacBio RS II Sequencer or the Oxford Nanopore MinION, making it easier to assemble whole genomes from scratch without the use of a reference genome. Bio-IT World previously covered MHAP following a presentation by senior author Adam Phillippy at the PacBio User Group Meeting this June; however, the newly released paper features much greater detail, including assemblies of the human genome and four important model organisms.

    As the price of DNA sequencing continues to fall, the barrier to analyzing genomes is increasingly the compute power needed to make sense of the raw sequencing data. This is especially true for de novo whole genome assembly, where software tools have typically fallen back on the brute force, computationally demanding method of comparing every DNA fragment's entire sequence against every other fragment in search of overlaps.

    MHAP, created by Konstantin Berlin and Sergey Koren, instead renders each fragment as a short series of numbers generated through hash functions. Briefly, every DNA fragment fed into MHAP is split into a string of k-mers (the authors recommend 16-mers for a human genome). Each k-mer is then subjected to the same series of hash functions, which output short numbers. For each hash function, only the smallest of these numbers is stored to identify the DNA fragment. Thus, the fragment is reduced to one number for each hash function, and each of those numbers corresponds to a single k-mer. MHAP can then search for fragments with many of the same identifying numbers, indicating

  • this_isnt_my_real_alias this_isnt_my_real_alias Aug 29, 2014 12:05 PM Flag

    Monday the market is closed....Labor Day.

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 27, 2014 10:16 AM Flag

    And ZERO debt .

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 26, 2014 1:11 AM Flag

    Because from 1:18 EDT to 2:52 EDT there was zero trades, then at 2:52 on large volume (for this stock) it shot right up to $3.12 from $2.38.

    Look on the one day chart you can see this. All of a sudden there was excitement over VII for some reason. One trade alone was for almost 25,000 shares, very unusual for this one.

    There was a buy of 24,700 shs. at $3.04, followed by a buy of 11,100 shs. at $3.12, then a dip back to $2.92, followed with a buy of 13,500 shs. at $3.1499.

    And those buys were between 2:59 to 3:12 EDT, that is big volume buys all of a sudden for a very thinly traded stock, there were more after those ones, but those buys really stuck out to me.

    This will be interesting to see why ?

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 25, 2014 11:55 PM Flag

    Something is going on here.

    Very unusual volume today in regular, and after hours........with good appreciation.

    Something coming up ?

    Anybody know ?

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 21, 2014 9:30 AM Flag

    Increase sales in a BIG way for DGLY ?

    To be successful in the stock market you have to have the capability to recognize a ground floor opportunity when it is staring you in the face.

    Right now DGLY is a PERFECT example

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 21, 2014 9:15 AM Flag

    And will be mushrooming.

    "Following a fatal shooting by police officers of an unarmed teenager that sparked violent protests in Ferguson, a Californian mayor has called for police officers to use body-worn cameras. An ordinance will be introduced at next week’s council meeting that will mandate this practice. The incident has ignited riots all over the city and led to people calling for immediate action.

    An article by The Wall Street Journal highlights the benefits of police officers wearing cameras that record everything that occurs between police forces and citizens. It also elaborates on how, during the first year since the cameras were introduced, the use of coercion by cops declined 60% and citizen complaints against the police dropped 88%. Although it is not known how many police departments in the state are currently following the new practice, it is still considered as a potential way to curb fatal incidents, such as those that occurred in Ferguson."

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 21, 2014 9:05 AM Flag

    No doubt about it. The real world isn't Mayberry, a town whose sherriff doesn't carry a gun.

    The cost for supplying cameras to cop cars and body cameras is pennies compared to losing one lawsuit against police.

    In many instances a small town can be bankrupted in losing just one lawsuit settlement.

    The cameras are INSURANCE, you wouldn't drive your car, live in your home, or even breath (health insurance) without it.

    What has recently ocurred is a loud and clear WAKE UP CALL !

    Nobody's WORD can be totally believed, from either side ..............visual PROOF is a needed back-up, simple as that.

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 18, 2014 4:18 PM Flag

    When the police do not have make hand-written police reports......just plug and go.

  • this_isnt_my_real_alias this_isnt_my_real_alias Aug 15, 2014 1:22 AM Flag

    zordiac They can not issue stock for more than the market price. Who would pay more for the stock than what it is trading at ?

    Now I'm going to take a guess.......this is from the PR....."The offering is subject to market conditions, and there can be no assurance as to whether or when the offering may be completed, or as to the actual size or terms of the offering."

    My guess is that there may be some kind of positive announcement in the near future, and they may wait for a better stock price to actually issue the shares.

    I'm guessing that based on their statement that ....."The offering is subject to market conditions".

    And based on the fact they announce a new share offering......but no amount of shares or price ? Usually it's all announced at the same time.

    If a positive announcement appears, then they've already announced a new share issuance and they can just go ahead and do it.

    But like I said, I think they want to wait for a more lucrative price.

    The higher the price the less shares they have to issue.

    We'll just have to wait and see.

  • this_isnt_my_real_alias this_isnt_my_real_alias Aug 14, 2014 5:05 PM Flag

    I doubt if they will issue much more than 2 M shares. Maybe even less, or a little more, but definitely not 10 M shares.

    My brother previously had NEO, and when they issued more shares the stock initially dropped, but recovered very fast. I see an opportunity coming, and I will try and lower my average price. I've kinda been hoping for this, even though I'm already very long here.

    He also was/is waiting for this opportunity. The initial expected response is panic, but in reality here, this is an opportunity.

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 14, 2014 4:20 PM Flag

    Will raise profitability.

    They didn't say how many shares or what price yet .

  • this_isnt_my_real_alias by this_isnt_my_real_alias Aug 8, 2014 4:18 PM Flag

    It's under : NeoGenomics Cancer Diagnostics Reference Laboratory. The 5:24 min. video is in there.

  • Reply to

    NEO support falling?

    by dg41510 Aug 5, 2014 4:26 PM
    this_isnt_my_real_alias this_isnt_my_real_alias Aug 5, 2014 8:01 PM Flag

    dg4510 You are psyching yourself out. If you are going to be concerned with every tick of the stock you really should take the pressure of yourself and sell and move on.

    You are putting too much emphasis on the small picture, you need to look at the bigger picture here,

    Many people today look at stocks like it's a video game instead of an investment, if you look at any chart of any stock in an instant you will see that there was not a moon shot, the whole stockmarket is totally about psychology, the old fear/greed thing.

    The business that NEO is in is not going away, or not going out of style, not a fad, if management thinks things are looking up we have to take them at their word and just be patient to be rewarded.

    Look beyond the daily fluctuations, and try and realx. Things will all fall in place.

    The only absolute correct comment anybody can make about a stock will fluctuate....Guaranteed !

    You have to be able to live with that, or be out of it.

  • this_isnt_my_real_alias by this_isnt_my_real_alias Jul 25, 2014 6:31 PM Flag

    But that's understandable, that's what makes a stock-market.

    10,000 More shs. for me today, and may take more if the price gets any better than today.

    But I think today was the bottom, or real close to it IMO.

    Still the same company, that could possibly be scooped up.

  • Out of the clear blue......Like Allen Funt used to say,....."somewhere, sometime, when you least expect it......WHAM !

    Surprise !

  • this_isnt_my_real_alias by this_isnt_my_real_alias Jul 23, 2014 4:12 PM Flag

    167,648 Shares at the ASK of $ 500 shares for good measure.

  • Reply to

    The Motley Hypesters Strike Again....

    by semanresu Jul 22, 2014 5:56 PM
    this_isnt_my_real_alias this_isnt_my_real_alias Jul 23, 2014 12:39 AM Flag

    Hey semanresu If you are not aware that those "writers" for MF, and SA are nothing but glorified message board posters, you must be really new to this game.

    I previously had another stock that one of the posters on that message board was an "article writer" for SA, and he would divulge on the message board what his next article was going to be.

    And within two days his "article" would appear.

    Stevie Wonder can see right through their amateur garbage.

    I once found a site while googling, for SA hiring "writers" for them, working from your own home for $20 an hour. I never could figure how they could figure how many hours they worked and could prove it.

  • Reply to

    Big Ol Nasty Rejection Candle

    by milkman92714 Jul 22, 2014 3:33 PM
    this_isnt_my_real_alias this_isnt_my_real_alias Jul 22, 2014 3:41 PM Flag

    Hey milkman, you've clearly proven you know not what you are talking about with your "50" day slap down comment, so I suggest you cease and desist from further embarrassing yourself.

    Or, if you choose, continue on as the message board clown.

    Every board has at least one.

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