Ole Gunnar Solskjaer’s side are looking to avoid any further embarrassment after their dire 2-0 defeat against Burnley last weekend – their first loss to the Clarets at Old Trafford since 1962.
Solskjaer and Ed Woodward have come under intense pressure this month, with supporters launching protests and leaving before the final whistle, while the club are still haggling with Sporting over a fee for midfielder Bruno Fernandes.
Tranmere sit 60 places below United in all and have won just one of their last 10 League One fixtures. However, they defeated Watford 2-1 to reach this stage of the competition and will be hoping to force another upset.
Here is everything you need to know:
When is it?
The match will kick-off at 3pm on Sunday 26 January.
How can I watch it?
The game will be broadcast live on BT Sport 1, with coverage beginning at 2.30pm.
Tranmere: Davies, Ridehalgh, Monthe, Morris, Ferrier, Jennings, Caprice, Perkins, Blackett-Taylor, Clarke, Danns
Manchester United: Romero, Dalot, Lindelof, Maguire, Jones, Shaw, Matic, Pereira, Lingard, Martial, Greenwood