Micky Mellon’s side upset Watford in the previous round, defeating Nigel Pearson’s side 2-1 in their FA Cup replay. However, Rovers have picked up just one win in their last 10 League One games.
Ole Gunnar Solskjaer’s side are looking to avoid further embarrassment after their dire 2-0 defeat against Burnley last weekend.
Fans streamed out en masse and called for Ed Woodward’s dismissal during the match and Solskjaer will hope his academy graduates – with Mason Greenwood likely to lead the line against Tranmere - can return some good feeling to the club.
Here is everything you need to know:
When is it?
The match will kick-off at 3pm on Sunday 26 January.
How can I watch it?
The game will be broadcast live on BT Sport 1, with coverage beginning at 2.30pm.
Tranmere: Davies, Ridehalgh, Monthe, Ferrier, Morris, Jennings, Caprice, Perkins, Blackett-Taylor, Clarke, Danns
Manchester United: Romero, Dalot, Lindelof, Maguire, Jones, Shaw, Matic, Pereira, Lingard, Martial, Greenwood